Yield and quality of tobacco are dramatically decreased and tobacco chemistry altered when weeds are not managed. Today herbicide weed control in tobacco is necessary for a safe, efficient and economic production. The main objective of this work was to evaluate and identify herbicides already used in other crops, that are efficient in weed control, and selective on tobacco. Results are presented from field trials, with herbicides used alone (thiazopyr, EXP 03316, benfluralin, oxyfluorfen, EPTC) or in combination (thiazopyr + pebulate, thiazopyr + pendimethalin, pendimethalin + oxyfluorfen), and were compared with the control herbicide combination pebulate + pendimethalin. EXP 03316 was applied as PRE, pebulate, pendimethalin, benfluralin as PPI, thiazopyr and ETPC as PPI and PRE, and oxyfluorfen as PRE and POE. The data collected were on herbicide effectiveness and phytotoxicity (selectivity), yield, quality and chemical composition of cured leaves. Phytotoxicity was evaluated as tobacco plant fresh weight at 30 and 60 days after transplanting. No phytotoxicity was observed for any of the herbicides used in the trials. All the herbicides, at the rates and soils used in the trials, were selective. Weed control with certain herbicides (ETPC, oxyfluorfen, thiazopyr + pebulate), was as good or better compared to control herbicide combination pebulate + pendimethalin. Yield and quality of cured leaf was not affected compared to control herbicide.