Tobacco smoke contains thousands of chemical compounds, including polycyclic aromatic hydrocarbons (PAHs). PAHs are a class of compounds composed of two or more fused benzenoid rings. A method was developed and validated for the determination of naphthalene, pyrene and benzo[a]pyrene in heated tobacco products (HTP) by UPLC/Fluorescence. HTP products were aerosolized using a Cerulean SM450 20-port linear smoke machine under ISO and Health Canada intense smoking parameters. Total particulate matter was collected on Cambridge filter pads, extracted in acetonitrile and analyzed on an UPLC instrument equipped with a fluorescence detector. Chromatographic separations were performed using a Waters Acquity BEH Shield RP18 1.7 µm, 2.1×50 mm column and a gradient of water/acetonitrile solvent system. Naphthalene, pyrene and benzo[a]pyrene are detected at excitation/emission wavelengths of 277/330, 334/374 and 364/408 nm, respectively. The method was reproducible with average relative standard deviations of less than 8%. The accuracy was within 100±4% for both smoking regimes. The results of the method validation will be reported.