Currently no international, European or national Standards in the field of toxicology of additives in tobacco products exist. The Directive 2001/37/EC of the European Parliament concerning the regulation of tobacco products asks for a statement of additives in tobacco products and its toxicological evaluation. The national implementation of this Directive in Germany (the Tobacco product regulation) was the reason for the elaboration of a guide by the DIN working group "Toxicology of additives". The base material for toxicological methods have been compiled and it was evaluated under which basic conditions already existing methods are transferable to the field of tobacco. Not only the toxicological analysis itself but also the subject matter of the preparation and treatment of samples prior to the actual test for ingredients has to be considered. This guide "DIN Technical Report 133 - Toxicological Evaluation of Additives for Tobacco Products - A Guide" is regarded as a basis for the elaboration of future technical regulations in the form of standards. And it is of importance for the industry as well as for the national inspection bodies in the federal states because it serves as an aid for the preparation and evaluation of toxicological data for additives in tobacco products. This poster gives an overview of the present state of the art in this field and the first attempts for a test strategy described in the guide.