Acetic acid, octanal, and raspberry ketone are common flavour additives in e-liquid. This study mainly focused on the storage stability of the three flavour components under different temperatures and illumination conditions. The study was carried on in simulated e-liquid , in which the concentrations of acetic acid, octanal and raspberry ketone were all 500 ppm, percentage concentration of nicotine was 5 % and the mass ratio of PG: VG was 1:1. According to the guideline of ICH (The International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use), three kinds of storage stability study conditions were set as 25 °C with light, 25 °C in darkness, and 40 °C in darkness, and the sampling times were 0d, 5d, 10d, 15d, 1M, 3M, and 6M. GC-FID and GC-MS were applied as quantitative detection methods for the three target compounds. The contents of 3 analytes under 25 °C-light had no significant differences compared with those under the condition of 25 °C-darkness, while decreased by 11.62 %, 99.19 %, and 56.46 %, respectively after 6 months of storage at 40 °C-darkness. The results of linear regression analysis at 25 °C-darkness condition showed that the stable period of octanal and raspberry ketone was 30 days and 42 days, respectively, while the content of acetic acid remained at the relatively stable level after six months of storage. The illumination had no effect on the stability of the three flavours in the simulated e-liquid but the high-temperature could accelerate the process of esterification, acetalization and ketalization reaction. The storage stability of flavour substances in e-liquid has been explored preliminary in this study, which provided a theoretical basis and guidance for further research on the e-liquid shelf life.