Investigations were made with four parent tobacco varieties of different types (Prilep P-23, Prilep P 8-9/80, Floria FL-7 and Samsun S-1) in the role of mother, and MV-1 variety in the role of father, and their four F₁ hybrids for mode of inheritance for morphological properties of the leaves. The crossings were made in 2017, and the experiment with the parent genotypes and their hybrids was set up in 2018, in a field trial at the Scientific Tobacco Institute – Prilep, in a randomized block design with four replications. All appropriate cultural practices were applied during the growing season.

The aim of this work was to study the mode of inheritance of the number of leaves per stalk and the length, width and area of the leaves in the middle belt, in the offspring of the first generation.

The results of the studies indicate the fact that the obtained hybrids did not show a heterotic effect. Intermediate and partial dominance in inheritance is an indicator of good successive selection of individuals in future generations and rapid fixation and stabilization of the studied traits. All crosses (P-23 × MV-1, P 8-9/80 × MV-1, FL-7 × MV-1 and S-1 × MV-1) are rich and interesting selection material.