Six inorganic anions including F-,Cl- , NO2- , Br- , SO2-4 and NO3- in tobacco leaf samples were extracted by ultrasonic wave with deionized water at 60 °C for 30 minutes, separated on Dionex AS18 column, with IonPac AG18 as guard column and ASRS-ULTRA II as regenerative depressor, using the solution of 23mmol/L KOH as eluent (flow rate: 1.00mL/min) , and detected by conductivity detector. It was found that the recoveries of the method were between 95.0% and 105.0% , the relative standard deviation was 2.63% , the correlation coefficients of linear regression equations were from 0.9940 to 0.9988 and the detection limits of the anions were in the range of 0.01 to 0.061,µg/mL.