Tobacco Science & Technology, 2011, 10, p. 65-69., ISSN.1002-0861
Development and preliminary application of one-step RT-PCR kit for detection of tobacco CMV
1. Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, P.R. China; 2. China Tobacco Hunan Industrial Co., Ltd., Changsha 410007, P.R. China
To detect tobacco CMV quickly and accurately, a pair of specific primers was designed according to the relatively conservative region in the CMV genomic sequence, and enzymes and buffers needed for reverse transcription and PCR were integrated. By...