Tobacco specific N-nitrosamines (TSNAs) are known components of mainstream cigarette smoke. Quantification of TSNAs from tobacco smoke has traditionally required time-consuming sample cleanup and lengthy sample analysis times. As an alternative to traditional GC-TEA analysis, we have developed an LC/MS/MS method that requires no sample cleanup and has an analysis time of less than 10 minutes. This procedure involves an aqueous extraction of a mainstream smoke sample from a Cambridge filter pad. The pad is spiked with deuterated internal standards, shaken for one hour, filtered and then the sample is analyzed by LC/MS/MS. This method provides a rugged procedure that maximizes sample throughput and assures data reliability. The extraction procedure exhibits excellent performance with all trapping media spikes and laboratory fortified matrix spikes having relative recoveries of 95-105%. In addition, this method has limits of detection that are substantially lower than traditional methods with detection limits <0.05 ng/ml for each of the TSNAs. Considering the rapid analysis time and the lack of sample cleanup, this method allows for the analysis of over 100 mainstream smoke samples for TSNAs within a 24-hour period.