Volatile nitrosamines (VNAs) are classified as group 2A and 2B carcinogens in humans by the International Agency for Research on Cancer (IARC), have been associated with increased incidences of carcinogenesis and have been linked to lipid peroxidation, oxidative stress, and insulin resistant diseases. Potential sources of VNA exposure include contaminated water, cosmetics, food products, as well as tobacco products. Literature has shown that smoking cigarettes or using smokeless tobacco products may cause higher exposure to VNAs compared to other common sources. As a result, the U.S. Food and Drug Administration listed multiple VNAs as Harmful and Potentially Harmful Constituents (HPHC) in tobacco products and tobacco smoke. Previously reported methods of VNA quantitation used complex protocols that required large sample sets of cigarettes, tobacco smoke collection using impingers, and using treated smoking pads, which causes decreased sample throughput. Additionally, previous methods utilize a non-specific method of detection (gas chromatography-thermal energy analyzer), which may affect VNA quantitation. We report the development and validation of a specific and sensitive method for the detection of 8 VNAs (N-nitrosodimethylamine (NDMA),N-nitrosodiethylamine (NDEA), N-Nitrosomethylethylamine (NMEA), N-nitrosodipropylamine (NDPA), N-nitrosomorpholine (NMOR), N-nitrosopyrrolidine (NPYR), N-nitrosopiperidine (NPIP), and N-nitrosodibutylamine (NDBA)) in mainstream tobacco smoke using Tedlar® bag smoke collection, one-step SPE sample cleanup, and GC-MS/MS for quantitation. Method validation was completed for both ISO 3308 (IS) and Canadian Intense (CI) smoking regimens and the method was applied to 20 commercial brands of cigarettes and little cigars. Method precision and accuracy was 5-28% RSD and 84-111%, respectively, and the limit of detection (e.g., NDMA, 0.931ng/cig (ISO) and 0.693 (CI); NMEA 0.0267 ng/cig (IS) and 0.0279 (CI)) was improved for most analytes compared to previously reported methods.