K326 is an American flue-cured tobacco variety with resistance to root-knot nematodes, moderate resistance to granville wilt and tolerance to alternaria brown spot. It also produces leaf with a sugar to nicotine ratio which is desired by the cigarette manufacturers. Like most American varieties, K326 has no resistance to white mould. Most of the locally bred varieties while possessing this white mould resistance do not have the desirable leaf quality characteristics of K326. A breeding programme was therefore initiated to incorporate the good attributes of K326 into locally bred flue-cured varieties. In the year 2000/2001 season, selected F7 lines were evaluated for yield quality attributes. Twenty-three F7 breeding lines that were selected during the 1999-2000 season for their sugar and nicotine content in addition to their field appearance were tested in a 5 x 5 simple lattice with two replications at Mwimba Research Station. Each gross plot consisted 24 plants with an intra and inter-row spacing of 0.6 and 1.2 m respectively while the net plot had 20 plants. AF36-01, AF36-04, AF36-06, AF31-02, AF38-02 and AF38-01 were the five top yielding lines and had also the highest gross returns. Two of these lines, AF38-01 and AF38-06, had also large leaf area. While all these five lines showed no reaction to the alternaria brown spot at Mwimba, three of them, AF36-06, AF36-01 and AF38-02 showed moderate susceptibility to nematode root galling with an average score of 2.