Research is in progress at Fattoria Autonoma Tabacchi of Città di Castello (Perugia, Central Italy), to investigate the possibility to use natural and biological treatments, instead of the ordinary chemical ones, to enhance tobacco early growth in the field, by improving early plant phosphate uptake. Growers of Central Italy, where the tobacco growing season is relatively short, are very concerned about low soil phosphate availability in their sub-alkaline soils. This problem is increased by progressively reduced phosphate applications, due to higher P fertilizer costs, and the so-called agro-environmental measures, which affect P allowed application rate. Natural acidifying sweet chestnut-tannin, applied as in-the-row placed treatments or by microirrigation, proved to increase P availability in sub-alkaline soils. These applications have also an interesting nemastat side effect, which is under study mostly on vegetable crops and tobacco in Northern Italy. Some tests have been carried out for the last two years also on VA mycorrhizae, Trichoderma spp. and rhizosphere bacteria based formulations, applied soon before field transplanting to the trays of transplant seedlings, and at field tobacco transplanting, to enhance early plant nutrition and resistance to abiotic stresses and root diseases. These investigations have indicated that these natural and biological treatments offer potential benefits to tobacco growers, especially where the agro-environmental measures are applicable. A decreased dependency upon fertilizer agrochemicals improves tobacco sustainability, and helps reduce crop environmental impact.