The aim of this paper is to study the quantitative relationship between above-ground nitrogen accumulation (ANA) of flue-cured tobacco and canopy spectral parameters, and then build a monitoring model for ANA of flue-cured tobacco based on various spectral indices. Tobacco was grown in the field under different nitrogen fertilisation levels. Canopy spectra data at different growing stages was collected and biomass and nitrogen content in different parts of plants was determined. The correlation analysis of ANA of flue-cured tobacco and multiple spectral parameters showed that nitrogen accumulation increased as nitrogen fertilisation level increased, meanwhile, nitrogen fertilisation level too low or too high would prompt nitrogen transfer from leaf to stem and root. The correlation between ANA and canopy reflectance was significantly negative (P<0.05) at visible band (460-710 nm) and positive at band (760-1300 nm). Leaf nitrogen accumulation (LNA) was highly correlated with ANA, and quantitative equations were established between spectral parameters and ANA, and monitoring models on ANA were established using canopy. Spectral parameters, such as ratio vegetation index (RVI (680,810)), difference vegetation index (DVI (680,810)), normalised difference vegetation index (NDVI (680,810)), enhanced vegetation index (EVI810), soil-adjusted vegetation index (SAVI) and optimisation of soil adjusted vegetation index (OSAVI), all could be used to predict ANA, and the prediction accuracy R2 were 0.901, 0.792, 0.839, 0.929, 0.921, 0.930 respectively, and root-mean-square error (RMSE) were 0.264, 0.245, 0.193, 0.188, 0.202, 0.260 respectively. Regression analysis of the relation between red edge amplitude (DλRed) and ANA of flue-cured tobacco showed good inversion, in which the regression equation was y =8.376x2.0901 RMSE=0.197. It could be concluded that ANA in flue-cured tobacco could be monitored effectively by key vegetation indices, especially EVI810, OSAVI and DλRed.