Bacterial wilt (BW) is a devastating soil-borne bacterial disease caused by Ralstonia pseudosolanacearum. Many efforts have been made to control BW, however there is still a lack of effective, green and safe measures. Bacteriophages are expected to become an important biocontrol resource for prevention and control of the latest generation of wilt diseases. Many bacteriophages infecting R. pseudosolanacearum have been isolated at present, however, the bacteriophages are still insufficient to bio-control the R. pseudosolanacearum which presents much diversity in the environment. In this study, a lytic bacteriophage PQ43 infecting R. pseudosolanacearum was isolated from the rhizosphere soil of tobacco in Ningxiang, Changsha, China. Transmission electron microscopy and genomics analysis indicated that PQ43 belonged to the family Siphoviridae, order caudoviridaes. The genomics with a full length of 47,156 bp and a mean G+C content of 64.27 %, contains 74 open reading frames (ORFs), and does not contain tRNA, resistance or virulence genes. Bacteriophage PQ43 also exhibited high temperature stability (< 60 °C, 2 h), strong pH tolerance (pH 3-10), and strong UV tolerance (< 110 min). A pot experiment showed that the addition of bacteriophage could effectively control tobacco BW, especially the bio-control effect of PQ43 application in 108 PFU/ml and 107 PFU/ml treatments by root irrigation, and could reduce the incidence of tobacco BW significantly (to 92.80 % and 58.84 %, respectively), within 30 days. So, bacteriophage PQ43 is considered as an effective candidate biocontrol agent to control tobacco BW disease caused by R. pseudosolanacearum.