Standardized smoking machine measurements of mainstream cigarette smoke constituents is a useful tool for product comparisons, but offers less utility for exposure assessments at the individual or population level. Exposure biomarkers measurements in clinical or large survey studies provide a much better means for directly assessing smokers’ exposure to select constituents. However, such measurements can require invasive techniques for collecting blood, urine, or other biological samples. One non-invasive technique to examine both usage and smoke intake is the analysis of spent cigarette filters which can be used to estimate the “mouth level intake”. A combination of qualitative analysis of mainstream smoking machine deliveries with the mouth level intake may provide useful information related to behavior and exposure. We have combined the analyses of biomarkers, mouth level intake, and smoking machine results to help understand how smokers use the products and how design features can help interpret biomarker results.