Systematic field and laboratory studies on soil and plants were conducted in the tobacco growing regions of Croatia from 2006 to 2008. The aim was to get better insight into the soil status and its influence on tobacco quality. In this paper contents of some heavy metals (Cd, Cr, Cu, Mn, Mo, Ni, Pb and Zn) found in different soil types and their accumulation in the tobacco leaf are presented. Soil and plant samples were taken from 72 locations, i.e., 72 family farms. Concentration of particular metals was determined by inductively coupled plasma optical emission spectrometry (Varian Vista MPX). Because of different physical and chemical soil characteristics and different parent material, the results are presented for six pedosystematic units. Comparison of concentrations of the investigated metal with allowable threshold values showed that all pedosystematic units of soils used for tobacco productions were not contaminated by heavy metals. Their concentrations were below allowable threshold values for soils in conventional and ecological agriculture. Content of particular metals in dry tobacco leaves is in the reference interval for this plant. Also, the low content of toxic metals, i.e. cadmium and lead, has to be emphasised.