Tobacco in an accumulator of heavy metals and this ability tends to differentiate among tobacco types and varieties. The objective of this study was to evaluate the levels of heavy metal concentrations in six varieties of Greek Oriental Aromatic tobacco type. Two field experiments were established at two locations of Northern Greece, namely Drama and Xanthi, in the spring of 1999. Six varieties of Oriental Aromatic tobacco type, namely BX 2/a, BX 81, BZ/7, N 34/4, B 84/31 and ZP 4/b, were cultivated for one growing season, in 4 replications. Five primings were collected and cured tobacco samples were analysed for heavy metal content. Quality index, gross and trade yield were determined and plant uptake was calculated from the gross yield and plant concentration. Selected soil parameters were also measured. Both soils were inorganic, acid, loams with small differences in the measured parameters. For both areas (Drama and Xanthi) among the varieties, gross and trade yield of ZP 4/b and N 34/4 were significantly the highest and B 84/31 the lowest. Quality index followed the opposite trend, although did not change drastically with respect to tobacco variety. Zinc and Mn concentrations in B 84/31 and BZ/7 were significantly the highest and ZP 4/b the lowest. Lead and Cd plant concentration and uptake were not significantly different among the 6 varieties studied.