Sucker control is a key process for tobacco production because it affects yield and quality. In general, sucker growth is suppressed by using a suckercide and the remaining suckers are removed by hand. However, such sucker management is a tough workload on farmers. In addition, the suckercide itself has environmental concerns. At present, some research papers on reducing suckers have been reported but their practical use has not been tested yet. In a previous CORESTA Conference, we already presented the three knock-out mutants (ls, bl, rev) suppressing sucker growth. The objective of this study was to evaluate agronomic traits and the effect of the target gene in a fewer sucker variety in a tobacco production area of Japan. The fewer sucker flue-cured Virginia cultivar with ls, F80, was developed using Coker 319 as a recurrent parent. This variety was cultivated and its characteristics measured in a production area of southern Japan. As a result, the secondary suckers of this variety were 20 % less than the check variety, Coker 319, and the tertiary suckers hardly appeared. F80 showed almost the same quality and agronomic traits as Coker319 and a higher yield tendency than Coker 319. It had dissected flowers; however, it was possible to produce 80 % of seed weight compared to Coker 319. F80 showed feasible agronomic traits to contribute to reducing workload and chemical use in Japan. We are planning to start commercial production in the 2023 crop. And breeding of new varieties with ls is being considered.