In 1983 one collection of breeding lines, maternal doubled-haploids, from crosses between several Burley cultivars, Kentucky type, with NC 107 (using N. africana technique for haploid production and culture in vitro of a piece of leaf midvein for chromosome doubling) were grown in the field. In the areas with higher incidence of viruses (PVY and TMV), 4 locations were established in 1984. From these plots plants were selected, based upon the degree of tolerance to the disease and plant type. As a check cultivar Ky 14 was used. A total of 11 lines were selected. In 1985 these 11 lines were grown again at the same locations in randomized blocks to evaluate their agronomic characteristics and yielding ability (kg/ha). From observations on the degree of tolerance to the viruses and yield, we have selected 5 lines (D-1021, D-1089, D-1125, D-1106 and D-1006) which possess cultivar potential.