For effectively decreasing the damage caused by viral diseases, improving the yield and quality of tobacco leaf, transplanting young seedlings under film was compared with conventional film mulching cultivation by field trials. The results showed that transplanting young seedlings under film effectively evaded the aphid’s migrating peak and decreased the probability of virus infection; comparing with control treatment, morbidity and incidence index decreased by 32 and 18.35 percentage points, respectively; and the control efficacy reached 76.84% in the years when viral disease was serious; while morbidity and incidence index decreased by 13.11 and 5.06 percentage points, respectively; and the control efficacy was up to 89.22% in normal years. Meanwhile, transplanting young seedlings under film could improve microenvironment in the field, make full use of natural resources, such as light, temperature, water, and promote the seedlings to grow vigorously. As a result, tobacco yield, the proportion of higher grade tobacco leaf, average price and output value increased by 22.3%, 12.17 percentage points, 10.7% and 35.3%, respectively; moreover, the tobacco presented better appearance quality and more harmonious chemical composition.