Soil acidity is a major growth-limiting factor for plants in many parts in the world. A three year field study during the period 1990-1992 was conducted on an acid soil representative of those where flue-cured tobacco is grown in the Agrinio alluvial plain, in Greece, to determine the effects of seven rates (0, 4, 6.5, 9, 11.5, 14 and 16.5 t/ha) of lime (CaO) on yield, quality and chemical composition of flue-cured tobacco and on certain soil properties and nutrient uptake. Randomized complete-block design was used with three replications. Liming significantly increased yield from 10 to 38% in the second and third year of lime application and improved the quality of flue-cured tobacco. Leaf Ca and Fe contents were higher in limed than in non-limed plots and increased with the amount of lime. Cured leaf from limed plots contained slightly lower Mg and K than that of non-limed plots. Leaf Mn content was significantly reduced in limed plots and remained almost stable as the applied lime increased, but liming did not affect Al concentration in cured leaf. Sugar content was reduced by liming in the first and the second year after lime application, but total N and nicotine were increased. Liming lowered the content ratios of reducing sugars/total N and reducing sugars/nicotine in the first and the second year after application but did not affect substantially the total N/nicotine ratio in cured leaf. Liming improved soil fertility and productivity and increased the yield without reducing quality of flue-cured tobacco.