It is well documented that snuff contains larger quantities of N-nitrosamines than other tobacco products. While the levels of tobacco-specific nitrosamines (TSNA) in the two leading moist snuff brands in the U.S. have gradually decreased over the last decade, a new moist snuff brand introduced in 1990 showed the highest concentrations of TSNA ever reported in the literature. Because of rising consumption of smokeless tobacco products and in view of the known biological properties of several TSNA, it is important to determine the cause underlying the phenomenon of increased TSNA levels in the new moist snuff product. Therefore a systematic study of the effect of adverse storage conditions under TSNA formation was undertaken. This study demonstrated that the TSNA profile was more significantly affected by manufacturing technology than by the effects of ageing during the tobacco storage.