Nornicotine accumulation in Burley tobacco is of concern because nornicotine is the precursor of the predominant Burley tobacco-specific nitrosamines (TSNAs), N'-nitrosonornicotine (NNN). However, screening of Burley tobacco is problematic because the conversion of nicotine to nornicotine is highly variable in Burley tobacco. Two genes have been identified which regulate the conversion or demethylation of nicotine to nornicotine. CYP82E4 mediates conversion of nicotine to nornicotine in the senescing leaves. CYP82E5v2 regulates nicotine to nornicotine conversion in the green leaves of tobacco. Point mutations were induced for both genes. The mutants have premature stop codons that terminate accumulation of functional gene products. These two mutants provide valuable tools to reduce nornicotine levels in tobacco leaves. The objective of this study was to develop molecular markers based on the single nucleotide polymorphisms (SNPs) between the two tobacco converter genes and their mutants. Four dCAPS (Derived cleaved amplified polymorphic sequence) markers were designed for gene CYP82E4. One dCAPS marker was identified for gene CYP82E5v2. These markers are gene-specific as polymorphisms reside in the gene sequences themselves and are predictive of the converter/non converter phenotype. In addition, since dCAPS are co-dominant markers, heterozygous and homozygous plants can be differentiated. The genotypes determined by the dCAPS markers were validated by DNA sequencing and phenotyping of the plants. These markers can be used in marker-assisted selection to quickly introgress the mutants into commercial varieties and reduce nornicotine levels in tobacco leaves.