Heated Tobacco Products (HTP) are designed to deliver a tobacco/nicotine-containing aerosol with significantly reduced toxicant exposure for users relative to combustible cigarettes. Polycyclic Aromatic Hydrocarbons (PAHs) are a diverse range of organic compounds containing two or more fused aromatic rings. PAHs are known inducers of drug-metabolising enzymes, which are present in cigarette smoke due to incomplete combustion of tobacco matter. Sixteen PAHs are included on the U.S. Food & Drug Administration (FDA) list of Harmful and Potentially Harmful Constituents (HPHCs) in tobacco products and tobacco smoke. FDA PMTA/MRTP application regulatory pathways for new tobacco products require manufacturers to submit robust product characterisation data to demonstrate a relative reduction in PAH toxicant exposure for smokers switching to “reduced-risk” HTP. We report the development and validation of a sensitive and selective gas chromatography with mass spectrometry (GC-MS) method for the quantitative determination of 17 PAHs in HTP aerosol. The work was partially based on a Hall Analytical in-house PAH method which has been successfully adapted for HTP aerosol analysis. Generated eHTP aerosols were trapped in 8-12 puff collections, 2 second durations, 55-mL puff volumes, 30-s puff intervals, bell curve profiles. The tobacco aerosolgeneration was performed on a Cerulean SM450e puffing machine set up for HTPanalysis with Cambridge filter pad aerosol collection. The chromatographic methodachieves limits of quantitation (LOQ) of 1 ng/mL (in solution) for Benzo[a]pyrene, witha working range of 1-250 ng/mL for target PAHs. The method has demonstrated a suitable degree of accuracy, precision, and robustquantitation. Initial experimental results obtained from commercially available eHTPdemonstrate a significant reduction in PAH levels compared with the literature datafor a 3R4F reference cigarette.