Pyridine-based nitrogen compounds are present in tobacco leaf and smoke. This study developed and validated a simultaneous analytical method for nitrogen compounds such as pyridine, 2-Picoline, 3-Picoline, 4-Picoline, 2,4-Lutidine, 2,5-Lutidine, 2,6-Lutidine, pyrrole, 3-Vinylpyridine and indole in mainstream smoke using gas chromatography-mass spectrometry (GC-MS). In validation of the analysis, nitrogen compounds were detected within 43 minutes using GC-MS and the correlation coefficient (R²) of the calibration curve was 0.999 or higher, showing high linearity. All 10 nitrogen compounds obtained good recovery (83 % ~ 110 %) and repeatability (1.1 % ~ 10.5 %). In addition, the compounds with higher molecular weight (MW) were collected more in the Cambridge filter pad than in the impinger and among the similar MW were affected by vapour pressure. This study shows that this method would be applicable for selected nitrogen compounds in mainstream cigarette smoke. Furthermore, it is expected that the method could be extended to the analysis of other nitrogen compounds.