Carbon sequestration is the expression used to define the process of removing carbon dioxide from the atmosphere, being carried out by plants during growth through photosynthesis. Therefore, vegetation has a great contribution to carbon capture and storage, however, the impact of agricultural crops on this process is not yet known. The aim of the present study was to determine the amount of carbon sequestered in Virginia-type tobacco plants throughout the plant's growth phase, under environmental conditions in Old Belt in Rio Grande do Sul, Brazil. Twenty-four tobacco plants (Virginia, variety PVH2343 sourced by Profigen do Brasil) were sampled in four different properties in Santa Cruz do Sul e Passo do Sobrado, following the usual cultivation processes (topping, 1^st, 2^nd, 3^rd, 4^th harvest) and subsequent removal of the rest of the plant (stem and roots). All samples were individually weighed (fresh matter) and then dried for 6 days at 70 °C in an oven to define the dry matter. All samples were ground separately for further analysis with a CHN Elemental Analyzer to determine the carbon concentration. It was observed that a Virginia-type tobacco plant has an average fresh weight of 1.85±0.44 kg, with an average of 157.53 g of fixed carbon, maintaining 36.88 % of its dry weight in carbon, that means, 8.5±0.94 % of its fresh weight. The highest concentration of carbon is found in the leaves of the last harvest (39.62 % of the dry matter), and the highest average volume in the roots with 54.68 g. With these data it will be possible to estimate the amount of carbon sequestered in tobacco plants/plantations, a fundamental basis for the analysis of the technological life cycle in the tobacco production chain.