Waterpipe tobaccos (shisha) are mixtures of glycerol, sugar syrups, and flavors with tobacco content ranging between 10 and 30% of total weight. Tobacco used in most products is flue-cured; however, products made with dark air-cured (DAC) tobacco are available. DAC products tend to have particles less than 5 mm in size and are quite fluid as the mixture of glycerol and sugar syrups is not well absorbed by such tobaccos. Thus, when we used with waterpipe tobacco holders (bowls) with holes in the bottom, we found leakage of the fluid into the stem the waterpipe (hookah) along with small particles of tobacco. Retail bowls with the same size and spacing of holes in the base as specified ISO 22486 (Waterpipe tobacco smoking machine – Definitions and standard conditions) gave an unacceptable loss of fluid and particles as the shishas were heated and puffed. However, not all shishas suffered the same amount of loss of fluid and small particles. The first step in understanding these differences was to obtain homogeneous samples from retail packages (200 or 250 g) of shisha. The fluid has partially separated from the tobacco in most packages. Thus, the entire contents of the package should be emptied into a 1-pint, wide-mouth, screw-cap jar. After capping, the jar was turned or rotated several times a day for at least two days prior to taking sample until there are no “wet” spots in the mixture. Then the following samples need to be taken: 30 g for the expressed fluids test, 50 g for the particle-size test, and 2 g for HPLC profiling of glycols and sugars and used immediately to prevent further separation.